Generator

Part:BBa_K3174007:Design

Designed by: iGEM19_Austin_UTexas   Group: iGEM19_Austin_UTexas   (2019-08-08)


Blue Fluorescent Protein with Medium Promoter and Weak RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created using Biobrick assembly methods.


Source

The parts used to make this Biobrick include BBa_K592100 (blue fluorescent protein), BBa_J23110- which is a medium Anderson Series promoter that has a measured relative strength of 844au- and BBa_B0031, which is a weak ribosome binding site with a measured relative strength of 0.13au. All of these subparts were obtained from the iGEM Distribution Kits.

References